Spectrophotometric Determination of Atorvastatin Clopidogrel and Rosuvastatin Using N-Brosuccimide and Congo Red Dye

ABSTRACT This study aimed at development a simple, accurate, sensitive and rapid spectrophotometric method for determination of Atorvastatin (Atro), Clopidogrel (Clo) and Rosuvastatin (Ros) drug in bulk sample and in dosage, by using N-Bromosuccimide (NBS) as oxidant. The method is based on oxidation of studied drug by addition of known excess (NBS) in acidic medium (5M) Hydrochloric acid. The reaction of (Atro) and (Ros) at 45ºC while the reaction of (Clo) at room temperature 25C°. followed by determination of residual (NBS) by reacting with fixed amount of Congo red dye, measuring the absorbance at 576nm, the amount of (NBS) reacted correspond to the amount of drugs and the measure absorbance was found increase linearly with the concentration of drug which is corresponds to amount of (Ator), (Clo) and (Ros) which is corroborated by correlation coefficient of 0.9986, 0.9982 and 0.999 for drugs respectively. The system obey Beer's law for 4-35, 4-32 and 4-35 µg/ml respectively. The molar absorptivity was 3.4104, 1.5104 and 3.5104 L. mol-1. cm-1, The limit of detection (LOD) and quantification (LOQ) were 0.131, 0.435, 0.143, 0.591 and 0.177, 0.433 µg.ml-1 for (Atro), (Clo) and (Ros) respectively. Comparable accuracy (3%) also the method was equally precise as shown by relative value (