Data from: An innovative ovipositor for niche exploitation impacts genital coevolution between sexes in a fruit-damaging drosophila

Limited attention has been given to ecological factors influencing the coevolution of male and female genitalia. The innovative ovipositor of Drosophila suzukii, an invading fruit pest, represents an appealing case to document this phenomenon. The serrated saw-like ovipositor is used to pierce the hard skin of ripening fruits that are not used by other fruit flies that prefer soft decaying fruits. Here, we highlight another function of the ovipositor related to its involvement in genital coupling during copulation. We compared the morphology and coupling of male and female genitalia in this species to its sibling species, D. subpulchrella, and to an outgroup species, D. biarmipes. These comparisons and a surgical manipulation indicated that the shape of male genitalia in D. suzukii has had to be adjusted to ensure tight coupling, despite having to abandon the use of a hook-like structure, paramere, because of the more linearly elongated ovipositor. This phenomenon demonstrates that ecological niche exploitation can directly affect the mechanics of genital coupling, and potentially cause incompatibility among divergent forms. This model case provides new insights towards elucidating the importance of the dual functions of ovipositors in other insect species that potentially induce genital coevolution and ecological speciation.Paramere images.zipContains image files used to perform elliptical Fourier analysis of the parameres in D. suzukii and D. subpulchrella.Video images of copulating Dsub_H243-1.zipContains video images (5 frames per second TIFF images) of copulating individuals. Conspecific pairs of male and female Drosophila subpulchrella (H243) .Video images of copulating Dsub_H243-2.zipContains video images (5 frames per second TIFF images) of copulating individuals. Conspecific pairs of male and female Drosophila subpulchrella (H243) .Video images of copulating Dsub_H243-3.zipContains video images (5 frames per second TIFF images) of copulating individuals. Conspecific pairs of male and female Drosophila subpulchrella (H243) .Video images of copulating Dsuz_TMUS8-1.zipContains video images (5 frames per second TIFF images) of copulating individuals. Conspecific pairs of male and female Drosophila suzukii (TMUS8) .Video images of copulating Dsuz_TMUS8-2.zipContains video images (5 frames per second TIFF images) of copulating individuals. Conspecific pairs of male and female Drosophila suzukii (TMUS8) .Video images of copulating Dsuz_TMUS8-3.zipContains video images (5 frames per second TIFF images) of copulating individuals. Conspecific pairs of male and female Drosophila suzukii (TMUS8) .Video images of copulating Dsub_H243xDsuz_TMUS8.zipContains video images (5 frames per second TIFF images) of copulating individuals.Heterospecific pairs of Drosophila subpulchrella (H243) females and Drosophila suzukii (TMUS8) males.Video images of copulating Dsub_H243disruptedxDsub_H243.zipContains video images (5 frames per second TIFF images) of copulating individuals.Conspecific pairs of disrupted Drosophila subpulchrella (H243) females (post-anal cuticle wounded) and intact D. subpulchrella (H243) males.Video images of copulating Dsub_H243_disruptedxDsub_H243.zipVideo images of copulating Dsuz_TMUS8disruptedxDsuz_TMUS8.zipContains video images (5 frames per second TIFF images) of copulating individuals. Conspecific pairs of disrupted Drosophila suzukii (TMUS8) females (post-anal cuticle wounded) and intact D. suzukii (TMUS8) males.Video images of copulating Dsuz_TMUS8_disruptedxDsuz_TMUS8.zipREAD MERead me file