Data from: The relationship between post-mating reproductive isolation and reinforcement in Phlox

The process of speciation involves the accumulation of reproductive isolation (RI) between diverging lineages. Selection can favor increased RI via the process of reinforcement, whereby costs to hybridization impose selection for increased prezygotic RI. Reinforcement results in phenotypic divergence within at least one taxon, as a result of costly hybridization between sympatric taxa. The strength of selection driving reinforcement is determined by the cost of hybridization and the frequency of hybridization. We investigated the cost of hybridization by quantifying post-mating RI barriers among Phlox species that comprise one of the best-studied cases of reinforcement. We determined if the strength of RI differs among lineages that have and have not undergone reinforcement, how much variability there is within species in RI, and whether RI is associated with phylogenetic relatedness. We found high RI for the species that underwent phenotypic divergence due to reinforcement; however, RI was also high between other species pairs. We found extensive variability in RI among individuals within species, and no evidence that the strength of RI was associated with phylogenetic relatedness. We suggest that phenotypic divergence due to reinforcement is associated with the frequency of hybridization and introgression, and not the cost of hybridization in this clade.Raw data used to estimate reproductive isolationThis spreadsheet contains the input files for the statistical analyses reported in the manuscript. Relevant abbreviations include "matSp" means "maternal species", "patSp" means "paternal species", "ct" means "cross type", and "sPerC" means "seeds per cross".Suni&Hopkins2018Evolution.xlsxBackcross GLMMsThis data file contains the R code used to assess whether seed set differed between conspecific crosses and backcrosses.backcrossGLMMs_forDryad.Rseed GLMMsThis data file contains the R code used to assess if heterospecific and conspecific crosses differed in seed set.seedGLMM_forDryad.Rgermination GLMMsThis data file contains the R code used to assess if germination of pure species seeds differed from hybrid seeds.germGLMMs_forDryad.RTotal RIThis data file contains the R code used to calculate total reproductive isolation among species pairs.GCIII_bootRI_USED_forDryad.RRI individual barriersThis data file contains the R code used to calculate reproductive isolation at the individual post-mating barriers.RI_individualBarriers_forDryad.RRI difference among species pairsThis data file contains the R code used to assess if species pairs differed in their reproductive isolation.RIDIfferenceAmongPairs_forDryad.