Hepatoprotective Properties of Cuminum cyminum Seeds Powder as Post-Treatment for Acetaminophen-Induced Injury

Abstract Background and Objective: Acetaminophen overdose can result in hepatic injury, mainly through oxidative stress. We investigated the protective effect of Cuminum cyminum (C. cyminum) seeds powder after acetaminophen administration. Materials and Methods: In this study, 30 male rats were allocated into five groups of six in number as follows: control, acetaminophen (A), and acetaminophen + C. cyminum 200, 400, and 800 mg/kg (A+C). After 24 hours of fasting, the control group received distilled water, and groups A and A+C received acetaminophen 1,000 mg/kg orally through gavage. Six hours later, the control group and group A were given distilled water, and groups A+C received C. cyminum 200, 400, and 800 mg/kg by gavage. Twelve hours after the second gavage, hepatic markers of oxidative stress and serum ALT and AST were assessed. Results: In group A, the activities of serum ALT and AST, the concentration of hepatic malondialdehyde and H2O2 increased, and peroxidase & catalase activities decreased substantially compared to the control group. C. cyminum administration in groups A+Cs resulted in the return of these changes toward group control. Conclusion: These results suggest that C. cyminum, due to its flavonoid and polyphenol contents, could diminish hepatic injury induced by acetaminophen. Keywords: Acetaminophen, Hepatotoxicity, Cuminum cyminum, Oxidative stress, Hepatic functio