MEMBRANE RESERVE OF LYMPHOCYTES IN MIDDLE-AGED PEOPLE

Age changes at the cellular level are closely related to the features of the functioning of biomembranes. In the present study the involvement of the membrane reserve of lymphocytes in middle-aged people in the mechanisms of regulation of the cell volume was studied. It was found that the functional activity of biomembranes depends on the elastic properties of its surface. It was proved that under the influence of adrenalin load in vitro the rigidity of the membrane increased and therefore the cell had limited resources for involving the surface pool of the plasmalemma in osmoregulatory reactions in the hypotonic environment. Reduction of the elasticity of the cell almost by 1.5 times led to an increase in the duration of the phase “regulatory volume increase” by 1.25 times in comparison with cells without load while the reserve of membrane structures involved to the volume regulation were decreased almost 2 times. Under the influence of the propranolol the stiffness of the membrane decreased by 1.25 times while the duration of the phase “regulatory volume increase” was decreased by 1.5 times but the percentage of additional membrane structures involved in the hypotonic environment decreased by 6 times compared to cells without load. The obtained data testify to participate of the elements of adenylate cyclases signaling pathways into the mechanisms of the lymphocyte’s volume regulation in the middle-age people by changing the elastic properties of cell’s surface. Key words: membrane reserve, lymphocytes, adenylate cyclases signaling pathways, elastic properties, middle-age people