COMPARATIVE PREDICTION OF PLASMA CONCENTRATION, BLOOD-BRAIN BARRIER PENETRATION, INTESTINAL ABSORPTION AND SKIN DIFFUSION OF IBUPROFEN AND ITS COMPLEX WITH COW'S GHEE IN SWISS ALBINO MICE

The aim of present investigation was to compare plasma, brain tissue concentration, intestinal absorption and skin diffusion of Ibuprofen per se and its 1:1 w/w optimized complex (formulation) prepared with cow’s ghee in Swiss albino mice. Ibuprofen per se and its complex prepared with cows ghee (studied in our previous work) in dose 300mg/Kg, were administered orally to Swiss albino male mice and comparison of concentration in plasma for first three hrs, obtained (by centrifuging of blood samples) and brain for first three hrs (by brain homogenization) as well as intestinal absorption (by cutting 1.75 cm segment of jejunum, filled with 5mg/0.5ml) and in-vitro skin permeability for 18 hrs (through 2 cm diameter excised epidermis (skin) of mice) were carried out and analyzed at UV spectrophotometer at λmax 264 nm. Ibuprofen 1:1 w/w proportion optimized complex has found to more bioavailable in plasma, significantly (p≤0.05) increase its concentration in brain, more facilitation through G.I tract as well as profound increase in in-vitro skin diffusion (revealed from flux and permeability coefficient value) as compare to Ibuprofen per se in Swiss albino mice. Such findings provide further understanding for the possible therapeutic effects of Ibuprofen per se and Ibuprofen 1:1 w/w proportion complex in further pre-clinical and clinical research