Estimation of Total Cholesterol in Ghee Prepared from Milk of Cows and Buffaloes

National Dairy Research Institute, Karnal (Haryana) (Manuscript received 31 May 1971; accepted 10 August 1971) Reinhold and Shiels' modification used in the estimation of cholesterol in blood has been successfully applied to estimate cholesterol in ghee. Twenty-six samples each of cow and buffalo ghee have been analysed for their total cholesterol content by this method and the data analysed statistically. Highly significant differences between the cholesterol levels of cow and buffalo ghee have been observed. Cow ghee contains higher levels (0.31%) of cholesterol than buffalo ghee (0.27%)