BIOCHEMICAL AND MOLECULAR IDENTIFICATION OF LISTERIA MONOCYTOGENES BACTERIA ISOLATED FROM RAW VEGETABLES IN MADHYA PRADESH, INDIA

Abstract The modern lifestyle pronounced changed eating habits around the world, today raw vegetables are used for major cravings but the packaging and storage conditions of this output can favor the growth of bacteria Listeria spp. or LM which is a foodborne pathogen. that's why it is necessary to check, they are difficult to identify and differentiate solely by biochemical tests and molecular methods. The aim of the present study is to explore. the had the identification of LM in various raw vegetables from wet and hypermarkets available in selected Madhya Pradesh districts using the different Biochemical test & 16s rRNA method. A variety of available vegetables (n = 312) consumed in the minimally processed states in Madhya Pradesh have examined ten types of selected raw vegetables for the presence of LM to found information on the presence of these organisms in these raw vegetables. In the years 2019-2020, a total of 147 of the 312 different raw vegetable samples collected tested positive for L. spp. or L. monocytogenes. Our findings demonstrated that the presence of L. spp. in selected vegetable products was generally medium, on average 47.11% in the years studied including LM