Data on assessing the effects of genetic divergence and morphology on Anolis lizard mating

The brown anole (Anolis sagrei) is a widespread neotropical lizard found on many islands in the West Indies as well as the coast of Central America. Across their range, brown anole populations show extensive ecomorphological trait variation and substantial genetic divergence. It is unclear, however, whether this genetic and morphological divergence results in reproductive isolation between populations. We investigated variation in mating behavior across populations by analyzing four hours of video of each of 234 captive male-female A. sagrei pairs encompassing all 36 possible pairings from six sampled islands. For each pair of individuals, we tested for an association between the occurrence of mating, morphological traits, and genetic relatedness of their populations. We found no support for the hypotheses that ecological and/or non-ecological divergence is driving premating reproductive isolation in A. sagrei. We did find that males with relatively short heads tend to mate more quickly and hypothesize potential explanations that warrant future investigation.The dataset "matings_dryad" is a CSV file containing outcomes of four-hour-long mating trials of pairings of Anolis sagrei and morphological data about the individuals used. The dataset "locations_dryad" contains sites of collection of populations. The dataset "island_sagrei_dryad.fasta" is a multi-FASTA file containing ND2 sequences from the individuals. The file "README_dryad" is a Word document that gives detail about these sequences. Missing morphological data in "matings_dryad" is due to some traits not being measured. All files are explained in the README_dryad file. Funding provided by: John Templeton FoundationCrossref Funder Registry ID: http://dx.doi.org/10.13039/100000925Award Number: 52287For the matings_dryad dataset: Mating data: Adult A. sagrei were collected in the summer of 2016 from the Bahamian islands of Abaco, Bimini, Conception, and Staniel Cay and from the Caymanian islands of Cayman Brac and Little Cayman in the Cayman Islands. Female A. sagrei, which can store sperm from previous matings and store sperm in the absence of fertilization, were kept isolated from males until