TO SCREEN AND ANALYZE THE ANTIMICROBIAL ACTIVITY OF TISSUES EXTRACT FROM HIBISCUS ROSA SINESIS

The present study has been design for the determination of antimicrobial activities from different tissues of Hibiscus rosa sinesis. It was determined against different microorganisms and to check different tissue extract against the phytochemicals activity such as flavonoids, tannins, saponin, glycosides, phenolic compounds, protein, amino acid, alkaloids and oils from the different tissues of Hibiscus rosa sinesis. Extracts showed increasing antibacterial property with increase in the extraction concentration. Maximum activity was observed in Enterobacter aerogenes against methanolic leaf extract of Hibiscus rosa sinesis at concentration 800mg/ml and it showed maximum zone of inhibition (59.3 mm), M. luteus showed zone of inhibition at 800 mg/ml concentration is (39 mm). Salmonella typhi in methanolic leaf extract show maximum zone of inhibition at 800mg/ml concentration with zone of inhibition 46.3 mm, while B. megatarium shows Minimum zone of inhibition 38.6 mm in leaf extract at same concentration i.e. 800mg/ml, Salmonella abony shows 24.8 mm inhibition zone at concentration 800mg/ml along with coccobacilli also shows some amount of antibacterial activity at concentration of 800 mg/ml with inhibition zone (35.3 mm) in leaves extract of Hibiscus. Microorganism B.subtilis, Pseudomonas aeuroginosa didn’t show any zone of inhibition in any different concentration it may be resistant to methanolic leaf extract of H.rosa sinesis