Changes Add support for input directly VCF (--vcf) files instead of FASTA or FASTQ files. These VCF files will be processed using the same normalization, phasing and annotation that we use for the full pipelines. If BAM files ((--bam and --bai)) are provided then the pipeline will also include technical annotations that allow to distinguish clonal from potential intrahost mutations. Add consensus FASTA derived from the VCF to the output folder. VCF normalization now runs before determining the consensus sequence for lineage determination. This has an effect on GATK Provide the reference file to mpileup right before call to ivar to reduce ivar calls as reported in #28 Phasing has been improved to avoid corner cases of merging overlapping i...
O. glaberrima reference genome based SNP calls. Filtering process described in paper
O. barthii reference genome based SNP calls. Filtering process described in paper
O. barthii reference genome based SNP calls. Filtering process described in paper
Major changes Support DNA assemblies in FASTA format as input instead of raw reads. Variant calli...
Major changes: Add coverage analysis for FASTQ pipeline Add Pfam domain annotations in the outpu...
Changes Fixes #26 and #41 by annotating VCFs with homoplasmic problematic sites from DeMaio. These ...
Changes Integrate pangolin for lineage determination. Integrate VAFator to annotate mutations with ...
A Nextflow pipeline for NGS variant calling on SARS-CoV-2. From FASTQ files to normalized and annota...
Major changes Add read trimming with fastp Output QC results in JSON format Integrate 4 variant...
Changes Added a custom step phasing of clonal mutations. All clonal mutations (ie: clonal/not clona...
Since its introduction in 2011 the variant call format (VCF) has been widely adopted for processing ...
O. barthii reference genome based SNP calls. Filtering process described in paper
O. barthii reference genome based SNP calls. Filtering process described in paper
O. barthii reference genome based SNP calls. Filtering process described in paper
O. barthii reference genome based SNP calls. Filtering process described in paper
O. glaberrima reference genome based SNP calls. Filtering process described in paper
O. barthii reference genome based SNP calls. Filtering process described in paper
O. barthii reference genome based SNP calls. Filtering process described in paper
Major changes Support DNA assemblies in FASTA format as input instead of raw reads. Variant calli...
Major changes: Add coverage analysis for FASTQ pipeline Add Pfam domain annotations in the outpu...
Changes Fixes #26 and #41 by annotating VCFs with homoplasmic problematic sites from DeMaio. These ...
Changes Integrate pangolin for lineage determination. Integrate VAFator to annotate mutations with ...
A Nextflow pipeline for NGS variant calling on SARS-CoV-2. From FASTQ files to normalized and annota...
Major changes Add read trimming with fastp Output QC results in JSON format Integrate 4 variant...
Changes Added a custom step phasing of clonal mutations. All clonal mutations (ie: clonal/not clona...
Since its introduction in 2011 the variant call format (VCF) has been widely adopted for processing ...
O. barthii reference genome based SNP calls. Filtering process described in paper
O. barthii reference genome based SNP calls. Filtering process described in paper
O. barthii reference genome based SNP calls. Filtering process described in paper
O. barthii reference genome based SNP calls. Filtering process described in paper
O. glaberrima reference genome based SNP calls. Filtering process described in paper
O. barthii reference genome based SNP calls. Filtering process described in paper
O. barthii reference genome based SNP calls. Filtering process described in paper