CRISPR Gene Editing Assessment and Quality Control Using Next-Generation Sequencing

Derived from a prokaryotic adaptive immune system for cleavage of invasive nucleic acids, the CRISPR-Cas genome editing system, one of the most important breakthroughs in modern biotechnology, enhances the efficiency of DNA modifications in living cells (base and prime editing). CRISPR-Cas9 allows precise gene targeting and mutations in virtually any cell type and organism relying on cellular DNA repair pathways. One ongoing concern is that the CRISPR-Cas9 system induces mutations in the genome other than the target site. Meanwhile, off-target mutations can potentially disrupt the function or regulation of genes unpredictably. Therefore efficient and rapid strategies for genome editing validation are essential to facilitate the application of CRISPR-Cas9 for disease model conductions, gene and cell therapies, agriculture, bioenergy, and the development of more accurate CRISPR editing tools