ANTIOXIDANT PROPERTY ANALYSIS OF TOBACCO LEAVES IN AYURVEDIC FORMULATIONS

ABSTRACT The aim of this research is to determine H2O2 radical scavenging and total antioxidant activity (using Rutin) with water and ethanolic extracts of an Ayurvedic preparation of Nicotinatobacum(Black tobacco leaves). Latest use of tobacco leavesis in the field of neurodegenerative diseases such as Parkinsons disorder. In this study, ethanolic and water extracts were prepared from powdered N. tobacum leaves and its Ayurvedic formulations. Antioxidant activities were measured by Rutin method, H2O2 radical scavenging assays with UV-Vis spectrophotometer. In conclusion, the Ayurvedic formulations of N. tobacumhad significant H2O2 radical scavenging activity and total antioxidant activity. The results of this study show that the water and ethanol extracts of these alternative formulations of N. tobacumcan be used as easily accessible source of natural antioxidants and be used in the pharmaceutical industry. Keywords: Nicotinatobacum, Tobacco, Ayurveda, Antioxidant, Rutin, H2O2 REFERENCES Evans WC. Trease and Evans, Pharmacognosy. 14th ed. WB Saunders Company Ltd, London (1996). Pulok Mukherjee, Quality Control of Herbal Drugs: An approach to evaluation of Botanicals, 5th reprint (2012). SerhatKeser, SaitCelik, SemraTurkoglu, ÖkkesYilmaz and Ismail TurkogluHydrogen Peroxide Radical Scavenging and Total Antioxidant Activity of Hawthorn Keser et al / Chemistry Journal (2012), Vol. 02, Issue 01, pp. 9-12. Packer, L., Cadenas, E., and Davies, K.J.A. (2008) Free radicals and exercise: An introduction. Free Radical Biology and Medicine, 44, pp. 123-125. Jung, T., Höhn, A., Catalgol, B., and Grune, T. (2009) Age -related differences in oxidative protein-damage in young and senescent fibroblasts. Archives of Biochemistry and Biophysics, 483, pp. 127-135. Wells, P.G., McCallum, G.P., Chen, C.S., Henderson, J.T., Lee, C.J., Perstin, J., Preston, T.J., Wiley, M.J., and Wong, A.W. (2009) Oxidative stress in developmental origins of disease: Teratogenesis, neurodevelopmental deficits, and cancer. Toxicological Sciences, 108, pp. 4-18. Miller LP. Phytochemistry Organic Metabolites. Van Nostrand and Reinbold Company, New York (1973) 2:382-384 (8) Grinberg LN, Rachmilewitz EA and Newmark H. Protective effects of rutin against hemoglobin oxidation. Biochem. Pharmacol. (1994) 48: 643-649. Beretz A and Cazenave J. The effect of flavonoids on blood vessel wall interactions. In: Cody V, Middleton E, Harborne JB and Beretz A. (eds.) Plant Flavonoids in Biologyand Medicine II: Biochemical, Cellular and Medicinal Properties. Alan R Liss, New York (1988) 187-200. Ayurvedic Formulary of India, Volume I, Government of India, Department of Indian System of Medicine and Homeopathy p.359. Keser, S., Celik, S., Turkoglu, S., Yilmaz, O., and Turkog- lu, I. (2011) Determination of antioxidant capacities of ethanol and water extracts of AchilleamillefoliumL. (Yarow). Asian Journal of Chemistry, 23, pp. 3172-3176. Ruch, R.J., Cheng, S.J., and Klaunig, J.E. (1989) Preventi- on of cytotoxicity and inhibition of intracellular communi- cation by antioxidant catechins isolated from Chinese green tea. Carcinogenesis, 10, pp. 1003-1008. Indian Pharmacopeia, Controller of Publications, Government of India (1996) A-170. Jianxiong Yang, Juan Guo, JiangfengYaun, In vitro antioxidant properties of rutin, LWT- Food Science and Technology, Volume 41, Issue 6, July 2008, p1060- 1066. FatemehFathiazad, Abbas Delazar, RoyaAmiriand Satyajit D. Sarker, Extraction of Flavonoids and Quantification of Rutin from waste Tobacco Leaves. Article 12, Volume 5, Number 3, Summer 2006, p 222-227. Science and Technology, Volume 41, Issue 6, July 2008, p1060- 1066