Changes in some haematological parameters induced on exposure to a sub-lethal dose of fluoride (NaF) in the freshwater fish, Channa punctatus (BLOCH)

ABSTRACT Alterations in some haemato-biochemical parameters of a freshwater, air breathing fish, Channa punctatus under the stress of chronic, sub-lethal dose (10ppm) of fluoride (NaF) for 75 days were studied. The TEC, haemoglobin % and PCV steadily and significantly decreased, whereas the TLC was noted to increase significantly with an increase in the duration of exposure time. The MCV, MCH and MCHC decreased significantly with an increase in the exposure period. The plasma protein, lipid and glucose decreased progressively and significantly whereas the plasma cholesterol and triglyceride exhibited a significant increase upto 75days of exposure. The results indicate that fluoride in a chronic, sub-lethal dose of 10ppm brings about harmful alterations in different haemato-biochemical parameters, which can be used as a tool to assess the degree of pollution and the fish health. Key words: Fluoride (NaF), Channa punctatus, haemato-biochemical parameters, sub-lethal dose. REFERENCES Guney M, Oral B, Take G, Giray SG and Mugan T. Effect of fluoride intoxication on endometrial apoptosis and lipid peroxidation in rats, role of vitamins E and C. Toxicology. 2007; 7: 231-232. Azmat R. Natural Chemical technology for reducing fluoridation through chloride ions in fish of Sindh region. Jour. Chem. & Chem Engg. 2009; 3:57-63. Bhatnagar C and Regar BC. Neurogenerative effect of fluoride (NaF) on the brain of freshwater teleost, Labeo rohita. Ind. Jour. Environ. Sci. 2005; 9:15-19. Azmat R, Talat R and Khalid A. The length – weight relationship condition factor and impact of fluoride concentration in Johnius belangeris of Arabian Sea. Res. Jour. Enviorn. Toxicol, 2007; 1:138-143. Usha Rani O and Naik R. Sodium fluoride toxicity in fresh-water fish, Carassius auratus (Gold fish), Effets on the carbohydrate metabolism. Weekly Sci. Res. Jour. 2014; 1(50) : 1-5. Dhar V, and Bhatnagar M. Phsiology and toxicity of fluoride. Ind. Jour. Dent. Res. 2009; 20 (3) : 350-355. Camargo JA. Fluoride toxicity to aquatic organisms: A review. Chemosphere. 2003; 50 (3) : 251-264. Mariappan P, Yegnaraman V, and Vasudevan T. Occurence and removal possibilities of fluoride in ground waters of India. Polln. Res. 2000; 19 (2) :165 – 77. Saxena R, Gupta R, Tripathi M and Gopal K. Flouride induced haematological alterations in the freshwater fish Channa punctatus. Jour. Ecophysiol. Occup. Hlth. 2001; 1:139-146. Tripathi A, Kumar A, Rani A and Tripathi M. Flouride induced morphological and behavioural changes in freshwater fish Channa punctatus. Jour. Ecophysiol. Occup. Hlth. 2004; 4:83-88. Gupta R. Pathophysiological consequences to freshwater fish Channa punctatus induced by fluoride (Ph.D dissertation) Univ. Of Lucknow. 2003. Joshi PK, Bose M and Harish D. Changes in certain haemototogical parameters in a siluroid catfish, Clarias batrachus exposed to cadmium chloride. Pollut. Res. 2002; 21 (2) : 129-131. Shah SL. Haematological parameters in tench, Tinca tinca after short term exposure to lead. Jour. Appl. Toxicol. 2006; 26 (3) : 223-228. Ololade IA and Ogini O. Behavioural and haematological effects of Zinc on African Catfish, Clarias gariepinus. Intl. Jour. Fisheries Aquat. 2009; 1(2) : 22-27. APHA. Standard method of examination of water and waste water, 21st Ed.; American Publ. Health Asson. New York U.S.A. 2005. Shah SL and Altindag A. Haematological parameters of tench (Tinca tinca) after acute and chronic exposure of lethal and sub-lethal mercury treatment. Bull. Environ. Contam. Toxicol. 2004; 73:911-918. Shah SL and Altindag A. Alteration in the immunological parameters of tench (Tinca tinca) after acute and chronic exposure of lethal and sub-lethal treatments of mercury, Cadmium and lead. Turk. Jour. Vet. Anim. Sci. 2005; 29:1163-1168. Darbkin DL. Spectrophotometric Studies, XIV- The crystallo-graphic and optical properties of the haemoglobin of man in comparison with those of other species. Jour. Biol. Chem. 1946; 164: 703-723. Ochei J and Kolhatkar A. “medical laboratory Science-Theory and Practice”. Tata McGraw Hill Publishing Company, New Delhi. 2005; Pp. 281-283. Anonymous. Computerized Clinical Information System (CCIS). Denver Co. Micromedex Inc. Cooper GR and Mc Daniel V. “Standard method of clinical Chemistry”, 1970; Pp. 159. Adeyemo OK. Haematological profile of Clarias gariepinus (Burchell) exposed to lead. Turk. Jour. Fish and Aqua. Sci. 2007; 7:163-169. Maheswaran R, Debapaul A, muralidharan S, Velmurugan B and Ignacimuthu S. Haematological studies of a freshwater fish, Clarias batrachus (L) exposed to mercuric chloride. Intl. Jour. Integrative Biol. 2008; 2 (1) : 49-54. Ramesh M, Srinivasan R and Saravanan M. Effect of atrazinc (herbicide) on blood parameters of Common Carp, Cyprinus carpio (Actinopterygii: Cypriniformes). African Jour. Environ. Sci. Technol. 2009; 3 (2) : 453 – 458. Yaji AT and Auta J. Sub-lethal effects of monocrotophos on some haematological indices of Aftrican Catfish, Clarias gariepinus (Teugels). Jour. Fish. Intl. 2007; 2 (1): 115-117. Ishikawa NM, Ranjanipaiva MTT, Lombardi JV and Ferreira CM. Haematological parameters in Nile tilapia, Oreochromis niloticus exposed to sub-lethal concentrations of mercury. Braz. Arch. Biol. Technol. 2007; 50 (4) : 619-626. Alwan SF, Haidi AA and Shokar AE. Alteration in haematological parameters of freshwater fish, Tilapia zilli exposed to aluminium, Jour. Sci. & its Appln. 2009; 3 (1) : 12-19. Zaki SM, Moustafa S., Raseed H and Sharaf N. Effect of Vanadium toxicity on biochemical, haematological and clinical changes in Claias lazera present in the river Nile. American Eurasian Jour. Agric. Environ. Sci. 2007; 2(6) : 741 – 745. Martinez CBR, Nagae MY, Zaia CTB and Zaia DAM. Acute morphological and physiological effects of lead in the Neo tropical fish Prochilodus lineatus, Braz. Jour. Biol. 2004; 64 (4) : 797-807. Vutukuru SS. Acute effect of hexavalent chromium on survival, oxygen consumption, haematological parameters and some biochemical profiles of Indian carp, Labeo rohita. Intl. Jour. Environ. Res. Public health. 2005; 2 (3) 456 – 462