INVESTIGATION ON SYNERGISTIC ANTIBACTERIAL PROPERTIES OF SILVER NANOPARTICLES ENCAPSULATED WITH DIFFERENT STABILIZING AGENTS

The emergence and spread of antibiotic resistance is an alarming concern in clinical practice. This would lead to the development of new antibacterial agents from natural and inorganic substances. In the present scenario, nanomaterials are the leading requirements in the field of bionanotechnology and nanomedicine owing to their high surface to volume ratio. Hence the present study has been focused to evaluate the antibacterial activity of stabilized and non-stabilized silver nanoparticles (AgNPs) against selected bacterial pathogens. Silver nanoparticles were fabricated by chemical reduction method. The size of the particles was determined by X-ray diffraction pattern (XRD) and it ranges from 18-43 nm. The morphology of the particles was observed through scanning electron microscopy (SEM). Bactericidal effect of AgNPs was done by agar well diffusion method. Bacterial sensitivity was found to vary depending on the microbial species. The stabilized AgNPs showed excellent antibacterial activity (22 mm) to Streptococcus epidermis than the other experimental strains used. Tow way ANOVA test revealed that the function of AgNPs on bacterial pathogens is statistically significant. Therefore, our results suggest that chemically mediated silver nanoparticles could act as an effective alternative for the development of new antimicrobial agent to combat resistance problem