Data from: Microsatellite primers for the Pacific Northwest conifer, Callitropsis nootkatensis (Cupressaceae)

Premise of the study: Microsatellite primers were developed for Nootka cypress (Callitropsis nootkatensis) to provide quantitative measures for gene conservation that can assist in guiding management decisions for a species experiencing climate-induced decline. Methods and Results: Using multiplexed massively parallel sequencing, we identified 136,785 microsatellite-containing sequences from 489,625 paired-end 80 bp reads. After stringent filtering, we selected 144 primer pairs and screened variation at these loci in five populations of C. nootkatensis. Loci show between 3 and 36 dinucleotide repeats per locus, with an average of thirteen. Screening of these markers in the Pacific Northwest relative Chamaecyparis lawsoniana demonstrated no marker transferability. This finding highlights the narrow taxonomic utility of microsatellite markers in Callitropsis. Conclusions: These microsatellites show high polymorphism and can be used for routine screening of natural variation in Callitropsis nootkatensis, and will be particularly helpful in identifying clones and inbred relatives at the stand-level.Jennings_Nootkacypress_PopInfoThis comma-delimited file provides location and genotype data for individuals from three populations of Alaska and one population of Oregon Nootka cypress that were surveyed with 11 microsatellite loci. The file shows individual numbers, population names, population latitudes, population longitudes, and the microsatellite allele length for diploid loci. Missing genotypes are indicated by the value "0, 0".Jennings_NootkaCypress_PopInfo.csvJennings_Nootkacypress_PrimerSeqsThis comma-delimited file provides information for 144 primers that were screened on four Nootka cypress populations. Included are the primer name, forward and reverse primer sequence, the original sequence name from Illumina sequencing, and the nucleotide sequence of the microread (2 x 80 bp, with read 1 and read 2 separated by 8 N's).Jennings_NootkaCypress_PrimerSeqs.cs