Preclinical study of gastroprotective action of cryopreserved placenta extract

Koshurba I. V., Hladkykh F. V. Preclinical study of gastroprotective action of cryopreserved placenta extract. Abstracts of International scientific interdisciplinary conference «ISIC–2022»; 2022 November 17; Kharkiv, Kharkiv: Kharkiv National Medical University of the Ministry of Health of Ukraine. 2022. P. 88–89. Treatment and prevention of peptic ulcer of the stomach is not only medical but also social problem. As a potential gastroprotective agent, our attention was drawn to the cryopreserved extract of placenta (CEP), because the literature convincingly demonstrated that this cryoextract eliminates the ulcerogenic action of non-steroidal anti-inflammatory drugs, which has a mechanism similar to peptic ulcer disease. The aim is to conduct a comparative assessment of the severity of antiulcer activity of CEP in prophylactic, therapeutic and therapeutic-prophylactic regimens for use in the model of ethanol-prednisolone gastric lesions. Materials and methods of research. Studies were performed on laboratory male rats. To simulate damage to the gastric mucosa, rats were administered a single ethanol-prednisolone mixture: prednisolone (20 mg/kg) dissolved in 80.0% ethyl alcohol. In 24 h after administration of ethanol-prednisolone mixture, rats were removed from the experiment. CEP was used in three modes of administration: prophylactic – 1 time per day for 5 days before the administration of ethanol-prednisolone mixture; therapeutic – once in 60 minutes after administration of ethanol-prednisolone mixture; therapeutic and prophylactic – 1 time per for 3 days before the administration of ethanol-prednisolone mixture and 60 minutes after the administration of ethanol-prednisolone mixture. The effect of the studied drugs on the condition of the gastric mucosa was assessed macroscopically on a scale Yakovleva L.V. Results. Macroscopic evaluation of the condition of the gastric mucosa showed that after 24 hours. after administration of the ethanol-prednisolone mixture, the degree of damage averaged 3.7±0.37 points out of the five maximum possible points, and the ulcer index was 3.9 units from prophylactic five-day administration of esomeprazole resulted in an antiulcer activity of 45.9%, which was 1.8 times lower than that of the CEP. Erosive-ulcerative lesions of the gastric mucosa on the background of treatment-and-prophylactic modes of CEP were observed in only 28.6% of rats, which corresponded to the value of the ulcer index (0.3 units), and antiulcer activity was 92.3%. Under this regimen, the antiulcer activity of the CEP was practically comparable to the severity of the indicated activity of esomeprazole (97.4%). Conclusions. The administration of CEP in the treatment mode (1 time after ethanol-prednisolone mixture) is accompanied by the lowest antiulcer activity, which was 22.2%, which was 4.1 times lower than the effectiveness of esomeprazole. Therapeutic and prophylactic use of CEP led to a statistically significant (p