Dietary Aqua Fix Effectively Alters the Qualitative Protein Profile in the Head Kidney

Abstract The qualitative protein profile in head kidney of 6, 9 and 12 months old Labeo rohita was investigated by feeding with a diet mixed with an immunomodulator, Aqua Fix. 6 (group A), 9 (group B), and 12 (group C) months old fish (12-15 g wt.; 12 fish in each group) were fed with a diet mixed with Aqua Fix @ 50 mg/100 g of feed for 4 days. Another 3 groups (a, 6 months; b, 9 months; and c, 12 months) of same weight (12 fish in each group) were fed with a normal diet to act as controls for comparison. Head kidney showed significant alteration (with low and high molecular weight proteins) in the qualitative protein profile (analyzed by SDS page analysis), on day 1, 4, 7, 15 and 30 of experiment in all the three groups of 6 (group A), 9 (group B) and 12 (group c) months old fish. These results determine the effect of Aqua Fix in modulating the immune system of fish with regard to the marked changes in the profile of proteins. Keywords: Rohu. 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