ANALYSIS OF THE FUNGICIDE AND BACTERICIDE ACTIVITY OF MORINGA OLEIFERA AND PETIVERIA ALLIACEA EXTRACT ON MICROBIAL CROPS FROM PESTS INFECTING MANGIFERA INDICA

The fungicidal and bactericidal activity of Moringa oleifera and Petiveria alliacea leaf extract against pests affecting Mangifera indica was tested in vitro. The process of obtaining the extracts was carried out by maceration with ethanolic solvent followed by distillation with rotavapor at low temperatures to better preserve the plant's active principles, whose final product was packaged in amber bottles isolated from light. To identify mango pests, samples were taken with Stuart transport media followed by seeding and subsequent replication on nutrient agar in the case of bacteria using the streak seeding technique and Sabouraud agar for fungi using the puncture seeding technique. Three different concentrations of extract were made, varying between the weight of solute and the volume of solvent, being added to the agar using the disc-plate method, in which the extract was added to a filter paper disc in direct contact with the agar previously inoculated with the strain of interest. The diameter of the inhibition halo, if formed, was analyzed. Favorable results were obtained in the case of the 1:5 concentration, which formed inhibition halos in the two bacterial strains of interest. It is recommended to deepen the different extraction methods to determine the optimal process to obtain plant extracts to promote the use of fungicides and bactericides of organic origin