ANALYSIS OF CALCIFEROL AND ELEMENTS IN AQUEOUS EXTRACT OF MUNTIGIA CALABURA FRUIT USING LC-MS/MS AND ICPMS TECHNIQUES

Muntigia calabura fruit have a lot of medicinal properties like antimicrobial, antioxidant, anti-inflammatory, and anticarcinogenic activities. It is used to reduce headaches, chronic pain, arthritis, blood pressure, prevent infection and boost immunity. This study planned to find out the amount of Calciferol, Cu, Zn and Selenium present in Muntigia calabura fruit. Aqueous fruit extract were prepared and analyzed by above mention tests using LCMS/MS and ICPMS analytical chemistry techniques. These both techniques are very advanced and assist to find out the accurate quantitative results. The sample preparation, analyzer method and calculations are carried out with reference to AOAC protocol. In this fruit extract contains 0.91µg/100g of Calciferol, 2.22mg/kg of Copper, 0.1mg/kg quantity of Selenium and Zinc metals were observed. Key words: Copper, Zinc, Selenium, LCMS/MS, ICPMS, Calciferol, Muntigia calabur