Growth responses of Chlorella sp. to some selected variants of culture medium and in effluents of a Brewery

Abstract: Global warming poses a major threat to our civilization that has led to unsustainable development worldwide. Though there are different strategies for global warming mitigation, carbon sequestration in water column via microalgae is the eco-friendly, cost effective and sustainable tool to capture and utilize carbon in a beneficial way. Microalgae, Chlorella sp. was cultured in ex situ using (a) standard basal medium as control, (b) culture medium aerated, (c) exogenously introduced liquid carbon dioxide into the culture medium. The growth of Chlorella was also examined under different pH (4 - 11) of the culture medium as well as in various dilutions (0, 25, 50%) of effluents of a Brewery under autoclaved and non- autoclaved conditions. There was no marked difference in growth of Chlorella sp. between 0 - 166 hours and between 218 - 272 hours either, but significant difference (P 0.05) among themselves. Chlorella sp. grown in brewery effluent showed the maximum growth in 50% and 25% dilution of non autoclaved and autoclaved effluent of beverage factory respectively suggesting that autoclaving saved 25% of bacteria driven nutrient demand