Primordial GATA6 macrophages function as extravascular platelets in sterile injury

Most multicellular organisms have a major body cavity that harbors immune cells. In primordial species like purple sea urchins, these cells perform phagocytic functions but are also crucial in repairing injuries. In mammals, the peritoneal cavity contains large numbers of resident GATA6+ macrophages, which may play a similar role. It is unclear how cavity macrophages suspended in the fluid phase (peritoneal fluid) identify and migrate towards injuries, however. Here, we show that cavity macrophages in fluid rapidly form thrombus-like structures in response to injury using primordial scavenger receptor (SRCR) domains. Aggregates of cavity macrophages physically sealed injuries and promoted rapid repair of focal lesions. In iatrogenic surgical situations, these cavity macrophages formed extensive aggregates that promoted the growth of intra-abdominal scar tissue termed peritoneal adhesions.Please start by reading the readme.txt file which contains all necessary information to use this data set. Funding provided by: Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen ForschungCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100001711Award Number: SNSF P1BEP3_181164Funding provided by: Deutsche ForschungsgemeinschaftCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100001659Award Number: DE 2654/1-1Funding provided by: Deutsche ForschungsgemeinschaftCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100001659Award Number: PE 2737/1-1Funding provided by: Natural Sciences and Engineering Research Council of CanadaCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100000038Award Number: RGPIN/07191-2019Funding provided by: Banting Postdoctoral Fellowships*Crossref Funder Registry ID: Award Number: Funding provided by: Canadian Institutes of Health ResearchCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100000024Award Number: Funding provided by: Canadian Institute of Health Research Fellowships*Crossref Funder Registry ID: Award Number: Funding provided by: Banting Postdoctoral FellowshipsCrossref Funder Registry ID: Funding provided by: Canadian Institute of Health Research FellowshipsCrossref Funder Registry ID:This data package contains a copy of the main data files exactly as used for the figures in this paper. Please refer to the Material and Methods section of our manuscript for a detailed description of how this dataset was collected and processed. In addition to the data shown in our figures, we provide the raw tif files used for the cell tracking and the respective R-Scripts used to visualize these tracking results. Data files are comma-separated values (csv) files except for flow cytometry data which are stored in a multi-sheet Excel file