EVALUATION OF ANTI-OBESITY ACTIVITY OF HERBALS FORMULATION USING HIGH FAT DIET INDUCED OBESE RAT MODEL.

The present study was designed to evaluate the anti-obesity activity of herbals formulation using high fat diet induced obese rat model. The obesity was induced using high fat diet. The study was carried out for 8 weeks and various biochemical parameters[cholesterol, HDL cholesterol, LDL, Triglycerides] and body weight were evaluated. On treatment with Ruta graveolens and Carica papaya 50mg/kg and 100mg/kg effectively showed decrease in body weight of obese rats when compared with disease control group. It was also observed that biochemical parameters i.e., Cholesterol, HDL cholesterol, LDL Triglycerides values werenormal in Ruta graveolens and carica papaya 50mg/kg and 100mg/kg treated groups when compared with disease control group. Histopathological studies were performed where disease control group showed presence of peri portal and peri biliary inflammation, moderate degeneration of hepatocytes and foci and central lobular necrosis was observed in hepatocytes of liver and treated groups of ruta graveolens and carica papaya showed similar results to standard group [orlistat 30mg/kg] where hepatocytes appeared normal in portal, periportal and central lobular region. Hepatic artery and portal vein appeared normal. Thus the results suggests that Ruta Graveolens and Carica Papaya possess anti-obesity activity. Keywords : Obesity, High fat diet, Body weight, cholesterol, HDL Cholesterol, LDL Triglycerides, Orlistat, Ruta Graveolens and Carica Papaya