Study of the effect of eugenol as a chemopreventive agent against induced hypercholesterolemia in rats

ABSTRACT Objectives: the study to detect the of the hypolipidemic effects of Eugenol in rats fed. Methods: this study was designed to detect the chemopreventive effect of Eugenol against induced hypercholesterolemia in rats twenty female rats divided in four group. the first group included cholesterol and eugenol administration cholesterol 1% in diet and Eugenol 10 mg/kg B.W orally the second group Eugenol administration Eugenol 10mg/kg B.W orally. The third group cholesterol administration cholesterol 1% in diet the fourth control group administration distal water and normal diet. antioxidant assessment, and immunohistochemistry were measured. Results: A significant (P0.05) between Eugenol and Control groups. There was also a significant (P0.05) difference when compared with each other, and non-significant between Eugenol and Control. The result of Immunohistochemical stain in liver of rat that feeding on high cholesterol diet ( 1%) shows the level of SREBP-1 protein expression in the hepatocellular cytoplasm. The tissue shows high expression of SREBP-1 protein in the liver . While the result of Immunohistochemical stain in liver of rat that feeding on high cholesterol diet (1%mg B.W) and Eugenol (10%) in parallel shows the level of SREBP-1 protein expression found in hepatocellular cytoplasm with moderate expression of SREBP-1 protein in the liver. The result of Immunohistochemical stain in liver of rat that administrating Eugenol orally (10%) only shows the level of SREBP-1 protein expression found in hepatocellular cytoplasm. The result of histopathological study also showed the section in the liver of rats that feeding on high cholesterol diet (1%mg B.W). The tissue shows severe fat droplet infiltration in the hepatocytes cytoplasm (fatty degeneration/fat infiltration. The results also showed histopathological section in the liver of rats that feeding on high cholesterol diet (1%mgB.W) and orally administration of Eugenol (10%) in parallel. The tissue shows mild fat droplet infiltration in the hepatocytes cytoplasm (fatty degeneration / fat infiltration. The result of eugenol histological section in the liver of rats that orally administration of Eugenol (10%). The tissue shows normal texture of hepatic tissue and normal hepatocytes without any significant occupied lesion (SOL). The animals are feeding on normal fat diet in this group. The result shows normal texture of hepatic tissue and normal hepatocytes without any SOL. Conclusions: The study concluded that kisspeptide 20 nmol /animal of kisspeptid by s/c every 72 hour for 30 days show effect in normal restoring of some reproduction hormones concentrationKeywords: Antioxidant, Hypercholesterolemia, Eugenol, MDA, SREBP-