Fig. 1 in Stilbenes: Source plants, chemistry, biosynthesis, pharmacology, application and problems related to their clinical Application-A comprehensive review

Fig. 1. Phylogenetic tree of known stilbene-producing plants: Each family was marked with a different color in the figure. The circle-marked species are capable of producing stilbenes. Kappaphycus striatum (JN897024.1) and Silvetia siliquosa (JF718409.1) are included as out-groups. The phylogenetic tree was constructed using the ITS2 (ribosomal internal transcribed spacer 2) genus sequence. ITS2 sequences were obtained from NCBI (National Center for Biotechnology Information) (https://www.ncbi.nlm.nih.gov/). Some unannotated sequences were identified and delimited based on Hidden Markov models (HMMs), which was performed through the online website-ITS2 Database (http://its2.bioapps.biozentrum.uni-wuerzburg.de/). The MUSCLE program in MEGA6.0 software was used to perform multiple sequence alignments, and the neighbor-joining (NJ) method was used to construct phylogenetic trees with 1000 bootstrap replicates. The kimura-2- parameter substitution model was also employed, and the confidence interval was 95%. The evolutionary tree is visualized and beautified by the online software iTOL (https://itol.embl.de/). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)Published as part of Teka, Tekleab, Zhang, Lele, Ge, Xiaoyan, Li, Yanjie, Han, Lifeng & Yan, Xiaohui, 2022, Stilbenes: Source plants, chemistry, biosynthesis, pharmacology, application and problems related to their clinical Application-A comprehensive review, pp. 1-28 in Phytochemistry (113128) 197 on page 7, DOI: 10.1016/j.phytochem.2022.113128, http://zenodo.org/record/823464