No water, no mating: Connecting dots from behaviour to pathways

Insects hold considerable ecological and agricultural importance making it vital to understand the factors impacting their reproductive output. Environmental stressors are examples of such factors which have a substantial and significant influence on insect reproductive fitness. Insects are also ectothermic and small in size which makes them even more susceptible to environmental stresses. The present study assesses the consequence of desiccation on the mating latency and copulations duration in tropical Drosophila melanogaster. We tested flies for these reproductive behavioral parameters at varying body water levels and with whole metabolome analysis in order to gain a further understanding of the physiological response to desiccation. Our results showed that the duration of desiccation is positively correlated with mating latency and mating failure, while having no influence on the copulation duration. The metabolomic analysis revealed three biological pathways highly affected by desiccation: starch and sucrose metabolism, galactose metabolism, and phenylalanine, tyrosine and tryptophan biosynthesis. These results are consistent with carbohydrate metabolism providing an energy source in desiccated flies and also suggests that the phenylalanine biosynthesis pathway plays a role in the reproductive fitness of the flies. Desiccation is a common issue with smaller insects, like Drosophila and other tropical insects, and our findings indicate that this lack of ambient water can immediately and drastically affect the insect reproductive behaviour, which becomes more crucial because of unpredictable and dynamic weather conditions.This data file contains mating assays data for the following parameters-copulations success, mating duration and mating latency, and water content. Four to five-day-old virgin Drosophila melanogaster flies were used for all the assays. Copulation success has been presented in number (number of pairs mated). Mating duration and mating latency are given in minutes and weight data is in milligrams. Funding provided by: Science and Engineering Research BoardCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100001843Award Number: SERB-SB/S2/RJN-129/2017Funding provided by: Canadian Natural Sciences and Engineering Research Council*Crossref Funder Registry ID: Award Number: RGPIN-2018–05551: Discovery GrantFunding provided by: Ahmedabad UniversityCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100015094Award Number: AU/SUG/DBLS/2017-18/03: Startup GrantFunding provided by: Canadian Natural Sciences and Engineering Research CouncilCrossref Funder Registry ID: Award Number: RGPIN-2018–05551: Discovery Gran