SKRINING DAN OPTIMASI PH DAN SUMBER KARBON KHAMIR OLEAGINOUS ASAL BREM DAN TUAK BALI DALAM MENGHASILKAN LIPID

Lipid merupakan senyawa organik yang dapat dimanfaatkan dalam industri makanan dan farmasi. Lipid organik dapat dihasilkan dari tumbuhan, hewan, maupun mikroorganisme. Salah satu mikroorganisme yang dapat menghasilkan lipid adalah khamir oleaginous. Penelitian bertujuan untuk mendapatkan isolat khamir koleksi UNJCC asal tuak dan brem bali dalam menghasilkan lipid, mengetahui identitas khamir, serta mengetahui pengaruh variasi sumber karbon dan pH dalam produksi lipid. Seluruh isolat dilakukan peremajaan dan di uji kualitatif menggunakan metode sudan III serta uji kuantitatif menggunakan metode NLMB (Nitrogen Limited Medium Broth), selanjutnya tiga isolat yang memiliki nilai kuantitatif tertinggi akan dilakukan identifikasi molekular berdasarkan daerah D1/D2 serta karakterisasi morfologi. Hasil peremajaan 16 isolat khamir asam brem bali dan 4 isolat khamir asal tuak bali diperoleh koloni dengan morfologi 85% mucoid-putih dan 15% butyrous-putih. Hasil skrining kualitatif didapatkan 100% isolat khamir memiliki warna kuning-orange setelah diuji menggunakan metode sudan III. Hasil skrining kuantitatif diperoleh 3 isolat yang memperoleh nilai persentase lipid tertinggi yaitu Vishniacozyma victoria UNJCC Y-89 (12,4±1,38%), UNJCC Y-85 (11,9±1,21%), dan Pichia kudriavzevii UNJCC Y-109 (11,5±1,92%). Hasil identifikasi diperoleh isolat UNJCC Y-85 teridentifikasi sebagai spesies Debaryomyces hansenii. Hasil pengujian nilai persentase lipid berdasarkan optimasi dengan 3 variasi pH (5, 6, 7), 3 variasi sumber karbon (glukosa, fruktosa, dan campuran glukosa fruktosa) dan jenis isolat Vishniacozyma victoria UNJCC Y-89, Debaryomyces hansenii UNJCC Y-85, dan Pichia kudriavzevii UNJCC Y-109) didapatkan persentase lipid tertinggi sebesar 60,68 ± 5,3% pada khamir Vishniacozyma victoriae UNJCC Y-89 di pH 5 dengan penambahan sumber karbon glukosa. ***** Lipids are organic compounds that can be used in the food and pharmaceutical. Organic lipids can be produced from plants, animals and microorganisms. One of the microorganisms that can produce lipids is oleaginous yeast. The aims of this study were to obtain yeast isolates from the UNJCC collection from tuak and brem bali in producing lipids, to determine yeast identity, and to determine the effect of variations in carbon sources and pH on lipid production. All isolates underwent rejuvenation and qualitative tests using the Sudan III method as well as quantitative tests using the NLMB (Nitrogen Limited Medium Broth) method. Furthermore, the three isolates that had the highest quantitative values were subjected to molecular identification based on the D1/D2 area and morphological characterization. The results of rejuvenation of 16 isolates of soursop brem bali yeast and 4 isolates of yeast from bali palm wine obtained colonies with a morphology of 85% mucoid-white and 15% butyrous-white. The results of the qualitative screening showed that 100% yeast isolates had a yellow-orange color after being tested using the Sudan III method. The results of the quantitative screening obtained 3 isolates that obtained the highest lipid percentage values, namely Vishniacozyma victoria UNJCC Y-89 (12.4 ± 1.38%), UNJCC Y-85 (11.9 ± 1.21%), and Pichia kudriavzevii UNJCC Y-109 (11.5 ± 1.92%). Identification results obtained isolate UNJCC Y-85 identified as a species of Debaryomyces hansenii. The results of testing the percentage value of lipids based on optimization with 3 variations of pH (5, 6, 7), 3 variations of carbon sources (glucose, fructose, and a mixture of glucose fructose) and the type of isolate Vishniacozyma victoria UNJCC Y-89, Debaryomyces hansenii UNJCC Y-85, and Pichia kudriavzevii UNJCC Y-109) obtained the highest lipid percentage of 60.68 ± 5.3% in yeast Vishniaco zyma victoriae UNJCC Y-89 at pH 5 with the addition of a carbon source of glucose