NILAI HEMATOLOGI MENCIT DIABETES YANG DIINDUKSI EKSTRAK DAUN KALIANDRA (Calliandra calothyrsus Meisn.)

Penderita diabetes akan mengalami perubahan nilai hematologi, dimana akan mengalami anemia, hiperinflamasi yang akan berpengaruh terhadap jumlah leukosit, dan produksi trombosit yang meningkat. Daun kaliandra (Calliandra calothyrsus Meisn.) mengandung metabolit sekunder sehingga berpotensi sebagai obat herbal terapeutik antidiabetes. Tujuan penelitian ini yaitu untuk mengetahui nilai LC50 ekstrak daun kaliandra dan nilai hematologi mencit diabetes yang diberi ekstrak daun kaliandra. Penelitian terdiri dari kelompok kontrol sehat, kontrol negatif (diabetes), kontrol positif (metformin 65 mg/kgBB), ekstrak daun kaliandra 140 mg/kgBB, ekstrak daun kaliandra 280 mg/kgBB, dan ekstrak daun kaliandra 560 mg/kgBB. Uji toksisitas ekstrak daun kaliandra dilakukan dengan metode Brine Shrimp Lethality Test (BSLT). Mencit diinduksi aloksan 210 mg/kgBB pada H1. Perhitungan kadar glukosa darah puasa (GDP) awal dilakukan pada H4. Pemberian ekstrak daun kaliandra dilakukan mulai H5 hingga H18. Perhitungan kadar GDP akhir, kadar hemoglobin, persentase leukosit, dan persentase trombosit dilakukan pada H19. Hasil penelitian menunjukkan bahwa nilai LC50 ekstrak daun kaliandra sebesar 4.576,29 ppm. Terdapat perbedaan signifikan pada kadar GDP awal dan akhir kelompok ekstrak daun kaliandra 140 mg/kgBB (Sig. 2-Tailed 0,05). Sementara itu, persentase leukosit dan trombosit berbeda nyata secara signifikan antar kelompok (Sig. < 0,05). Induksi ekstrak daun kaliandra pada mencit diabetes berpengaruh terhadap penurunan kadar GDP, penurunan persentase leukosit, dan penurunan persentase trombosit. ***** Diabetes will affect hematological values, which will cause anemia, hyperinflammation which will affect leukocytes count, and increased platelet production. Calliandra leaves (Calliandra calothyrsus Meisn.) contain secondary metabolites that have potential as antidiabetic therapeutic leaves herbal medicines. The purpose of this study was to determine the LC50 value of calliandra leaf extract and the hematological value of diabetic mice given calliandra leaf extract. The study consisted of a healthy control group, negative control (diabetes), positive control (65 mg/kgBW metformin), calliandra leaf extract 140 mg/kgBW, calliandra leaf extract 280 mg/kgBW, and calliandra leaf extract 560 mg/kgBW. The LC50 value of calliandra leaf extract obtained from Brine Shrimp Lethality Test (BSLT) method. Mice induced by alloxan 210 mg/kgBW on D1. Calculation of the fasting blood glucose (FBG) level before extract induction conducted on D4. Induction of calliandra extract leaves on D5 to D18. Calculation of the post FBG level, hemoglobin level, leukocyte percentage, and platelet percentage conducted on D19. The results showed that the LC50 value of calliandra leaf extract was 4.576,29 ppm. There was a significant difference in the pre and post FBG levels of the calliandra leaf extract 140 mg/kgBW group (Sig. 0.05). Meanwhile, the percentage of leukocytes and platelets differed significantly between groups (sig. < 0.05). Induction of calliandra leaf extract in diabetic mice resulted in a decrease in FBG levels, a decrease in the percentage of leukocytes, and a decrease in the percentage of platelets