FIGURE 1 from A Druggable FOXA1-Glucocorticoid Receptor Transcriptional Axis Drives Tumor Growth in a Subset of Non-Small Cell Lung Cancer

A subset of NSCLC cell lines is FOXA1 dependent. A, Scatterplot depicting FOXA1 gene effect, or degree of essentiality, as a function of its expression. Each dot represents a cell line. Data are curated from The Cancer Dependency Map (Broad Institute). Red text, breast cancer; blue text, prostate cancer; green text, lung cancer cell lines. B, Bar graph depicting FOXA1 gene effect in NSCLC cell lines as in A arranged in order of increasing FOXA1 expression. Positive gene effect values signify a proliferative advantage conferred by FOXA1 knockout, whereas negative gene effect values signify a proliferative disadvantage conferred by FOXA1 knockout. C, Immunoblot demonstrating knockdown of FOXA1 in Renilla and FOXA1 knockdown in NCI-H441 cells. D, Effect of FOXA1 knockdown by shRNA on cell proliferation in NSCLC. Datapoints are means ± SD, n = 6. Measurements are fluorescence of Resazurin at 590 nm taken on day 7 after plating. P value calculated by Student two-tailed t test (*, P P P P < 0.0001).