Additional file 1 of Mature human induced pluripotent stem cell-derived cardiomyocytes promote angiogenesis through alpha-B crystallin

Additional file1. Fig S1. Generation of the Akaluc-expressing hiPSC line. Fig S2. Flow cytometric analysis. Fig S3. qRT-PCR analysis. Fig S4. Immunocytochemistry. Fig S5. Comparison of Luc2- and Akaluc-expressing hiPSCs. Fig S6. Echocardiography. Fig S7. BLI. Fig S8. Assessment of the graft size and composition of hiPSC-CMs at 1 and 12 weeks post-transplantation. Fig S9. Cell proliferation in grafts at 4 and 8 weeks post-transplantation. Fig S10. Apoptosis in grafts at 4 and 8 weeks post-transplantation. Fig S11. Microvessels in grafts and in vitro assays for assessing angiogenesis by hiPSC-CMs. Fig S12. Angiogenesis profiler array and qRT-PCR. Fig S13. Expression of CRYAB in hiPSC-CMs. Fig S14. AAV-mediated CRYAB overexpression. Table S1. Primer sequences for genomic PCR and qRT-PCR in this study. Table S2. Antibodies used for immunohistochemistry. Table S3. Top 10 gene ontology (GO) terms enriched in D56-CM compared with D28-CM at 12 weeks after transplantation. Table S4. Top 20 genes with TPM-normalized counts upregulated by more than 4-fold in D56-CMs compared with D28-CMs before transplantation