Comparison of GreenGate 2.0 to GreenGate.
- Marcel Piepers (16955258)
- Katarina Erbstein (16955261)
- Jazmin Reyes-Hernandez (16955264)
- Changzheng Song (4182124)
- Tomas Tessi (16955267)
- Vesta Petrasiunaite (16955270)
- Naja Faerber (16955273)
- Kathrin Distel (16955276)
- Alexis Maizel (231067)
Publication date
September 2023
DOIAbstract
GreenGate 2.0 (GG2.0) expands the current GreenGate (GG)features set. GG2.0 offers an expanded repertoire of overhangs subdividing the existing GG A to E modules into 14 sub-modules. These modules can be cloned in a unique Universal entry vector to generate Level 0 entry clones. Up to 12 Level 0 clones can be assembled in one GG2.0 transcriptional unit compared to six for GG. Whereas GG only allowed the combination of two TUs to form a multi-gene Level 2 assembly, GG2.0 leverages GreenBraiding for the parallel, iterative assembly of a limitless number of TUs in Level 2 assemblies.</p
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