AvcID reduces the functionality of T7⁴¹² mutant phage.

Representative images of tenfold serial dilution plaque assays of T7WT (A), T7412 (B), or T7C74 (C) phages spotted on E. coli MG1655 expressing either active (top) or inactive (bottom) avcID system. Images are representative of three replicates. (D) CFU quantification of E. coli MG1655 over time in cultures indicated AvcID systems after infection with mutant T7412. (E) PFU quantification over time in cultures of indicated AvcID systems-containing cells infected with T7412. (F) Relative T7412 genome abundance comparing E. coli expressing pAvcID or inactive AvcID* over time. (G) Percent viable phage after infecting cells containing AvcID with T7412. Data represents the mean ± SEM of three biological replicate cultures. (H) Representative images of tenfold serial dilution plaque assays of T7WT (top) or T7412 (bottom) phages spotted on E. coli BL21 or BL21(D3E) expressing either active or inactive avcID system. Images are representative of three replicates.