International audienceThe catalytic function of lytic polysaccharide monooxygenases (LPMOs) to cleave and decrystallize recalcitrant polysaccharides put these enzymes in the spotlight of fundamental and applied research. Here we demonstrate that the demand of LPMO for an electron donor and an oxygen species as cosubstrate can be fulfilled by a single auxiliary enzyme: an engineered fungal cellobiose dehydrogenase (CDH) with increased oxidase activity. The engineered CDH was about 30 times more efficient in driving the LPMO reaction due to its 27 time increased production of H 2 O 2 acting as a cosubstrate for LPMO. Transient kinetic measurements confirmed that intraand intermolecular electron transfer rates of the engineered CDH were simila...
Biomass is a promising feedstock to reduce our dependence on fossil reserves. By using biomass, such...
International audienceThe many enzymes secreted by fungi to degrade plant biomass include a group of...
Lytic polysaccharide monooxygenases (LPMOs) are abundant in nature and best known for their role in ...
International audienceThe enzymatic conversion of plant biomass has been recently revolutionized by ...
A new paradigm for cellulose depolymerization by fungi focuses on an oxidative mechanism involving c...
The discovery of Lytic Polysaccharide Monooxygenases (LPMOs) has revolutionized our understanding of...
<p>Lytic polysaccharide monooxygenases (LPMOs) are powerful enzymes that oxidatively cleave glycosid...
The efficient depolymerization of lignocellulosic biomass to fermentable sugars by enzymatic hydroly...
Background: Enzyme-aided valorization of lignocellulose represents a green and sustainable alternati...
International audienceThe release of glucose from lignocellulosic waste for subsequent fermentation ...
International audienceLytic polysaccharide monooxygenases (LPMOs) are widely distributed in Nature, ...
Enzymatic degradation of plant biomass has attracted intensive research interest for the production ...
Lytic polysaccharide (mono)oxygenases (LPMOs) perform oxidative cleavage of polysaccharides, and are...
Oxidative processes are essential for the degradation of plant biomass. A class of powerful and wide...
Background: The ability of fungal cellobiose dehydrogenase (CDH) to generate H2O2 in-situ is highly ...
Biomass is a promising feedstock to reduce our dependence on fossil reserves. By using biomass, such...
International audienceThe many enzymes secreted by fungi to degrade plant biomass include a group of...
Lytic polysaccharide monooxygenases (LPMOs) are abundant in nature and best known for their role in ...
International audienceThe enzymatic conversion of plant biomass has been recently revolutionized by ...
A new paradigm for cellulose depolymerization by fungi focuses on an oxidative mechanism involving c...
The discovery of Lytic Polysaccharide Monooxygenases (LPMOs) has revolutionized our understanding of...
<p>Lytic polysaccharide monooxygenases (LPMOs) are powerful enzymes that oxidatively cleave glycosid...
The efficient depolymerization of lignocellulosic biomass to fermentable sugars by enzymatic hydroly...
Background: Enzyme-aided valorization of lignocellulose represents a green and sustainable alternati...
International audienceThe release of glucose from lignocellulosic waste for subsequent fermentation ...
International audienceLytic polysaccharide monooxygenases (LPMOs) are widely distributed in Nature, ...
Enzymatic degradation of plant biomass has attracted intensive research interest for the production ...
Lytic polysaccharide (mono)oxygenases (LPMOs) perform oxidative cleavage of polysaccharides, and are...
Oxidative processes are essential for the degradation of plant biomass. A class of powerful and wide...
Background: The ability of fungal cellobiose dehydrogenase (CDH) to generate H2O2 in-situ is highly ...
Biomass is a promising feedstock to reduce our dependence on fossil reserves. By using biomass, such...
International audienceThe many enzymes secreted by fungi to degrade plant biomass include a group of...
Lytic polysaccharide monooxygenases (LPMOs) are abundant in nature and best known for their role in ...