Determination of the concentration of thiocyanate ion in smokers’ blood using photo-spectroscopy

In this research, the concentration of thiocyanate ion in serum has been investigated, thiocyanate has negative effects on human body when it increasses higher than specific limit, this ion is produced by the metabolism of cyanide which exist in cigarette smoke. Spectrophotometric method has been used in the visible range at λ=460 nm, the formation of the complex between thiocyanate and reagent Ferric chloride (FeCl3) have been made in an ideal parameters such as temperature 25 ºC, time=15 min, pH=4.4, volume of citrate buffer=0.75 ml, volume of reagent (FeCl3) =1 ml and concentration of reagent = 0.02 mg/ml. The selected method showed good accuracy and precision, the accuracy of the method has been validated with R=99.3%, and the precision of relative standard deviation was (RSD % . The concentration of thiocyanate has been studied in a sample of 240 non-smokers’ and in a sample of 240 smokers’, these samples have been divided into different age groups. The maximum concentration of thiocyanate was 89.49 µmol/L in the fourth group (>50 years) of the non-smokers’, and the maximum concentration of thiocyanate was 190.27 µmol/L in the fourth group (>50 years) of the smokers’. The maximum concentration of thiocyanate was 91.647 µmol/L in the non-smokers’ males and 86.623 µmol/L for the non-smokers’ females, whereas the concentration of thiocyanate was 204.53 µmol/L in the male smokers’ and 167.33 µmol/L in the female smokers’