Transcription factors : a practical approach /

The pivotal role played by transcription factors in regulating gene expression has ensured that their study is a major area of current research activity. This book provides a comprehensive description of all the approaches and methods required to identify, isolate and characterize transcription factors. Starting with a specific DNA sequence, these protocols allow the detection and identification of proteins that bind to it and detailed analysis of the DNA binding site. This is followed by procedures for the functional assay of transcription factors and their purification. Also included are methods for the characterization of transcription factor genes, including DNA cloning. The analysis of transcription factors is of central interest to all molecular biologists involved in studying gene expression, making this book an important text for a wide range of researchers from postgraduate students to established scientists.Includes bibliographical references and index.The pivotal role played by transcription factors in regulating gene expression has ensured that their study is a major area of current research activity. This book provides a comprehensive description of all the approaches and methods required to identify, isolate and characterize transcription factors. Starting with a specific DNA sequence, these protocols allow the detection and identification of proteins that bind to it and detailed analysis of the DNA binding site. This is followed by procedures for the functional assay of transcription factors and their purification. Also included are methods for the characterization of transcription factor genes, including DNA cloning. The analysis of transcription factors is of central interest to all molecular biologists involved in studying gene expression, making this book an important text for a wide range of researchers from postgraduate students to established scientists