Determination of Arsenic by High Performance Liquid Chromatography Using a Transition Metal C18 Column

Arsenic, a metalloid, occurs naturally in drinking water and has become a major concern in recent years. There have been several reports on the analysis of arsenic utilizing high performance liquid chromatography (HPLC) with element specific detectors such as atomic absorption detector. The major goal of this research was to develop a method to analyze arsenic HPLC with UV detection. The success of the present research will help other laboratories that are not financially able to have the state of- the-art instruments. Our research focused on method development for the determination of As(ill) and As(V) using reversed-phase HPLC with a transition metal C 18 column. Due to the ionic nature of the arsenic species, and the non-polar packing material of the separation column, an ion-pairing reagent was included in the mobile phase. The mobile phase contained O.020M tetrabutyl-ammonium bromide and 30% acetonitrile. Validation of the methods such as linearity, detection limit, and reproducibility has been also performed