Assessment of BAP and NAA on In vitro Culture of Vanilla (Vanilla planifolia Andrews.) at The Final Stage of Subculture

The purpose of research was to examine BAP and NAA on in vitro culture of vanilla at final stage of subculture. The research was carried out in October 2021 – January 2022 at the Tissue Culture Laboratory of Balai Benih Tanaman Perkebunan, Salatiga. This research used a 3x4 factorial complete randomized design with 4 replicates. The first factor is the BAP concentration levels at 0, 1 and 2 mg/l. The second factor is the concentration of NAA at 0, 1, 2 and 3 mg/l. The parameters of initiation root period, number of roots, root length, shoot length and number of leaves obtained were analyzed for variance; if it had an effect, it was continued with DMRT. Data on the number of shoots was analyzed descriptively. The results showed an interaction between the additions of BAP and NAA on root length and number of leaves. The addition of BAP significantly affects the number of roots and shoot length, and the highest results were achieved at concentration of 0 mg/l. The NAA concentration had a significant effect on the initiation root period and shoot length that was best achieved at 0 mg/l.