Adad1 protein expression in adult ovaries and testes.

A-H: In the ovary, Adad1 is expressed in the cytoplasm of prefollicle stages through stage II oocytes (A and E) similar to the Ddx4/Vasa protein (B and G). Similar levels of Adad1 were seen in nuclei and cytoplasm of pre-follicle stage germ cells (E-H, arrows). Images in panels A-D and E-H are from different samples I-L: In the testis, Adad1is expressed in the cytoplasm of spermatogonia and spermatocytes but not in mature sperm (I), overlapping with Ddx4/Vasa expression (J). Magnification of the insets shows that Adad1 is both nuclear and cytoplasmic in single spermatogonia and spermatogonia in cysts of 2–4 cells (M-P, arrows). Scale bars: 200 μm for A-D and I-L, 20 μm for E-H.