Mouse weights in the Vehicle and treatment groups during the treatment phase of the study.

The diets for the Treatment groups were analyzed by LC/MS/MS to qualify the compound levels before the study and to determine compound level stability after the study. Diets for the (A) Vehicle group, (B) 30 mg/kg dose group, and (C) 40 mg/kg dose group were formulated to provide the correct dose based on average mouse weight and daily consumption. To determine the actual compound content in the diets, 2 pellets of feed were analyzed for each diet. Analysis of compound levels in the diet for the 30 mg compound/kg (mouse) dose showed that the compound concentration was 160 mg compound/kg diet prior to treatment, for an estimated dose of 32 mg/kg. After the study, the compound concentration was decreased to 114 mg compound/kg diet, for an estimated dose of 22.8 mg/kg. Analysis of compound levels in the diet for the 40 mg/kg dose had an initial concentration of 221 mg compound/kg diet, for an estimated dose of 44.2 mg/kg; after the study, the concentration was 160 mg compound/kg diet, for an estimated dose of 32 mg/kg.