Impact of mutation accumulation on the recognition of TSSAII^30-55 by Chagas disease sera.

Peptide arrays comprising sets of fully overlapped 15mers, encompassing residues 30 to 55 of TSSAII reference variant (TSSAII30-55) and bearing selected substitution(s) at polymorphic positions were probed with a pool of sera of chronic Chagas disease patients from Bolivia. The mean reactivity of each residue in the context of wild-type (top row) and single- or multiple-substituted sequences is indicated with a color scale. For each sequence, the replaced residue(s) is/are indicated. (TIFF)