<i>TSSA polymorphisms and antibody recognition</i>.

Peptide arrays comprising sets of fully overlapped 15mers, spanning residues 30 to 55 of TSSAII reference variant (TSSAII30-55) and bearing substitution(s) at polymorphic positions were probed with pools of chronic Chagas disease sera of different geographic origin. A) Reactivity of wild-type (wt) and single-substituted sequences for each pool. B) Mean reactivity of each residue in the context of wt (top row) or single-substituted sequences is indicated with a color scale. For each sequence, the replaced residue is marked in bold. C) Reactivity of sequences bearing combinations of either non-relevant (NR, green dots) or relevant (R, red dots) substitutions. Amino acid identity at the indicated positions in sequences bearing 4 relevant substitutions (for the Argentina pool sample) is shown in the boxes, where original and replaced residues are depicted in black and red, respectively. D) Reactivity of sequences bearing every possible combination of R and NR substitutions. For the Argentina pool sample, the identity of residues at positions 38, 39 and 40 from molecules displaying high (upper box), intermediate (middle box) or low reactivity (lower box) is indicated as in (C).