The role of reversed-phase liquid chromatography stationary phases in solvent-optimization procedures

It is increasingly important to develop liq. chromatog. (LC) sepn. procedures that meet high stds. of reliability and ruggedness. A no. of com. available software programs make method development less time consuming and less dependent on the knowledge of the analyst. Method development and the subsequent routine anal. generally start from the assumption of const. column selectivity. The authors study the column's role in reversed-phase LC method development. The differences in nominally identical stationary phases from various manufacturers have significant influences on the results of method development procedures. These differences also imply that the transferability of chromatog. sepn. conditions is poor between seemingly identical columns. [on SciFinder (R)