Symbols are frequently used to represent data objects in visualization. An appropriate contrast between symbols is a precondition that determines the efficiency of a visual analysis process. We study the contrast between different types of symbols in the context of scatterplots, based on user testing and a quantitative model for symbol contrast. In total, 32 different symbols were generated by using four sizes, two classes (polygon-and asterisk shaped), and four categories of rotational symmetry; and used three different tasks. From the user test results an internal separation space is established for the symbol types under study. In this space, every symbol is represented by a point, and the visual contrasts defined by task performance bet...