In a combined chemical biological and biophysical approach using time-lapse tapping-mode atomic force microscopy, we studied the partitioning of differently lipidated N-Ras proteins with various membrane-localization motifs into lipid domains of canonical model raft mixtures. The results provide direct evidence that partitioning of N-Ras occurs preferentially into liquid-disordered lipid domains, independent of the lipid anchor system. N-Ras proteins bearing at least one farnesyl group have a comparable membrane partitioning behavior and show diffusion of the protein into the liquid-disordered/liquid-ordered phase boundary region, thus leading to a decrease of the unfavorable line tension between domains. In addition, except for the monofar...
The spatial organization of lipids and proteins in biological membranes is of considerable interest....
Experiments have shown that homologous Ras proteins containing different lipid modification, which i...
H-, N- and K-ras4B are lipid-anchored, peripheral membrane guanine nucleotide binding proteins. Rece...
In a combined chemical biological and biophysical approach using time-lapse tapping-mode atomic forc...
AbstractIn a combined chemical biological and biophysical approach, we studied the partitioning of d...
In this study, two-photon fluorescence microscopy on giant unilamellar vesicles and tapping-mode ato...
AbstractRas GTPases play a crucial role in signal transduction cascades involved in cell differentia...
Cellular membranes are heterogeneous planar lipid bilayers displaying lateral phase separation with ...
Ternary lipid mixtures composed of cholesterol, saturated (frequently with sphingosine backbone), an...
The formation of domains in supported lipid membranes has been studied extensively as a model for th...
<div><p>Lipid-anchored Ras oncoproteins assemble into transient, nano-sized substructures on the pla...
Lipid-anchored Ras oncoproteins assemble into transient, nano-sized substructures on the plasma memb...
We present results from coarse grain molecular dynamics simulations of mixed model membranes consist...
AbstractThe special physical and functional properties ascribed to lipid rafts in biological membran...
Cell membranes contain a large number of different lipid species. Such a multicomponent mixture exhi...
The spatial organization of lipids and proteins in biological membranes is of considerable interest....
Experiments have shown that homologous Ras proteins containing different lipid modification, which i...
H-, N- and K-ras4B are lipid-anchored, peripheral membrane guanine nucleotide binding proteins. Rece...
In a combined chemical biological and biophysical approach using time-lapse tapping-mode atomic forc...
AbstractIn a combined chemical biological and biophysical approach, we studied the partitioning of d...
In this study, two-photon fluorescence microscopy on giant unilamellar vesicles and tapping-mode ato...
AbstractRas GTPases play a crucial role in signal transduction cascades involved in cell differentia...
Cellular membranes are heterogeneous planar lipid bilayers displaying lateral phase separation with ...
Ternary lipid mixtures composed of cholesterol, saturated (frequently with sphingosine backbone), an...
The formation of domains in supported lipid membranes has been studied extensively as a model for th...
<div><p>Lipid-anchored Ras oncoproteins assemble into transient, nano-sized substructures on the pla...
Lipid-anchored Ras oncoproteins assemble into transient, nano-sized substructures on the plasma memb...
We present results from coarse grain molecular dynamics simulations of mixed model membranes consist...
AbstractThe special physical and functional properties ascribed to lipid rafts in biological membran...
Cell membranes contain a large number of different lipid species. Such a multicomponent mixture exhi...
The spatial organization of lipids and proteins in biological membranes is of considerable interest....
Experiments have shown that homologous Ras proteins containing different lipid modification, which i...
H-, N- and K-ras4B are lipid-anchored, peripheral membrane guanine nucleotide binding proteins. Rece...