Investigating the development of native and iPSC- derived murine retinae using single cell RNA and ATAC-Seq

We report the generation and analysis of single-cell RNA-Seq data (> 38,000 cells) from mouse native retinae and iPSC-derived retinal organoids at four matched stages of development spanning the emergence of the major retinal cell types. We combine information from temporal sampling, visualization of 3D UMAP manifolds, pseudo-time and RNA velocity analyses, to show that iPSC-derived organoids broadly recapitulate the native developmental trajectories. However, we observe relaxation of spatial and temporal transcriptome control, premature emergence and dominance of photoreceptor precursor cells, and susceptibility of dynamically regulated pathways and transcription factors to culture conditions in organoids. We demonstrate that genes causing human retinopathies are enriched in cell-type specifying genes and identify a subset of disease-causing genes with expression profiles that are highly conserved between human retinae and murine retinal organoids. We generate bulk ATAC-Seq data for native and iPSC-derived murine retinae identifying ~125,000 peaks. We combine single-cell RNA-Seq with ATAC-Seq information and obtain evidence that approximately half the transcription factors that are dynamically regulated during retinal development may act as repressors rather than activators. We propose that sets of activators and repressors with cell-type specific expression constitute “regulatory toggles” that lock cells in distinct transcriptome states underlying differentiation.Investigating the development of native and iPSC- derived murine retinae using single cell RNA and ATAC-Se