In recent years, insufficiently characterised controls have been a contributing factor to irreproducibility in biomedical research including neuroscience and metabolism. There is now a growing awareness of phenotypic differences between the C57BL/6 substrains which are commonly used as control animals. We here investigated baseline metabolic characteristics such as glucose regulation, fasted serum insulin levels and hepatic insulin signalling in five different C57BL/6 substrains (N, J, JOla, JRcc) of both sexes, obtained from two commercial vendors, Charles River Laboratories (Crl) and Envigo (Env). Our results indicate systematic and tissue-specific differences between substrains, affected by both vendor and sex, in all parameters investig...
<p> Panel A shows representative Western blots from four DMSO-control and four wortmannin-treated ma...
Many metabolic studies employ tissue-specific gene knockout mice, which requires breeding of floxed ...
<p>Using both GC/MS and LDI-MS analysis, we found dramatic differences in female CHC profiles caused...
In recent years, insufficiently characterised controls have been a contributing factor to irreproduc...
In recent years, insufficiently characterised controls have been a contributing factor to irreproduc...
In recent years, insufficiently characterised controls have been a contributing factor to irreproduc...
In recent years, insufficiently characterised controls have been a contributing factor to irreproduc...
Open Access via the PLOS Agreement Funding: The authors received no specific funding for this work.P...
Basal glucose and serum insulin levels after a 5hr fast in female (A, C respectively) and male (B, D...
Representative images of western blots probed with antibodies for the detection of phosphorylated (p...
Body weight (A) of male and female animals belonging to five C57 substrains i.e., 6JCrl (black with ...
Objective The C57Bl/6J (Bl/6J) mouse is the most widely used strain in metabolic research. This stra...
Objective: The glucose tolerance test (GTT) is widely used in preclinical research to investigate gl...
(A) Female Abhd2KO mice showed a trend for increased plasma glucose at 15 and 30-minute timepoints d...
<p>Boxplots showing the smallest observation (lower bar), lower and upper quartile (box), median (li...
<p> Panel A shows representative Western blots from four DMSO-control and four wortmannin-treated ma...
Many metabolic studies employ tissue-specific gene knockout mice, which requires breeding of floxed ...
<p>Using both GC/MS and LDI-MS analysis, we found dramatic differences in female CHC profiles caused...
In recent years, insufficiently characterised controls have been a contributing factor to irreproduc...
In recent years, insufficiently characterised controls have been a contributing factor to irreproduc...
In recent years, insufficiently characterised controls have been a contributing factor to irreproduc...
In recent years, insufficiently characterised controls have been a contributing factor to irreproduc...
Open Access via the PLOS Agreement Funding: The authors received no specific funding for this work.P...
Basal glucose and serum insulin levels after a 5hr fast in female (A, C respectively) and male (B, D...
Representative images of western blots probed with antibodies for the detection of phosphorylated (p...
Body weight (A) of male and female animals belonging to five C57 substrains i.e., 6JCrl (black with ...
Objective The C57Bl/6J (Bl/6J) mouse is the most widely used strain in metabolic research. This stra...
Objective: The glucose tolerance test (GTT) is widely used in preclinical research to investigate gl...
(A) Female Abhd2KO mice showed a trend for increased plasma glucose at 15 and 30-minute timepoints d...
<p>Boxplots showing the smallest observation (lower bar), lower and upper quartile (box), median (li...
<p> Panel A shows representative Western blots from four DMSO-control and four wortmannin-treated ma...
Many metabolic studies employ tissue-specific gene knockout mice, which requires breeding of floxed ...
<p>Using both GC/MS and LDI-MS analysis, we found dramatic differences in female CHC profiles caused...