Statistics comparison (OrthoFinder output)

Insecticide resistance represents a major threat for the control of insect vectors. In last years, genetically-based tools have been proposed as a cutting-edge alternative to counteract this problem. However, high-quality -omics resources are crucial for an effective application of these tools. Here, we presented a de novo transcriptome of the mosquito vector Culex pipiens, assembled by using sequences of susceptible and diflubenzuron-resistant individuals. A total of 437,233,732 reads were assembled and annotated and the high-quality of the assemblage was confirmed by TransRate and BUSCO. A mapping percentage until 94.8% was obtained by aligning contigs to Nr, SwissProt, and TrEMBL, with 27,281 sequences that simultaneously mapped on the three databases. A total of 14,966 ORFs were also functionally annotated by using the eggNOG database. A comparison through the orthologues with other five mosquito species further validated the quality of the Cx. pipiens transcriptome. This resource represents therefore a valuable reference for further investigations about mosquito biology, their adaptation to insecticides, as well as to identify target sequences for genetic-based control tools.