RNASeq analyses of transcriptional changes induced by BCG vaccination reveal canonical biological pathways associated with protection.

Using transcriptional expression data derived from RNASeq, we performed analyses with Ingenuity Pathway Analysis software (IPA, Qiagen). Analyses focused on genes with a minimum of 1.5-fold change by RNASeq and a p-value 10 p-value for each canonical pathway, based on the number of representative genes in that pathway and the strength of the transcriptional changes. B) Bioprofiler analyses revealed disease associations or functional biological pathways predicted to be influenced by changes in gene expression. Bars depict the -log10 p-value for each disease or functional subset based on the number of representative genes in that pathway and the strength of the transcriptional change. Colors indicate the biological process superfamily that incorporates the noted individual pathways.