Experiment timeline.

Experimental design timeline Parent cultures were acclimated for 30 days at 26°C. At the start of the experiment an aliquote from the parent cultures was inoculated in fresh media to obtain the expected inicial cellular concentration, through dilution. From the new homogenate, parent cultures were aliquoted in triplicate bottles for each temperature and place at 26°C incubator. After 48h the first step increasing the temperature began. Following the increases in steps of 2°C every 48h, until they reached the final temperature of exposure. At 4 h and 72 h photosynthetic parameters were measured, and samples were taken for flow cytometric analusis and ROS production estimates. (JPG)