Development of analytical procedure for the quantitative validation of bronchospasmolitic thick extract from caucasian ivy

'Kavsuron' is a para-medical bronchospasmolitic preparation (on the base of thick extract) from the leaves of Hedera helix L. seu H. Caucasigena Pojark., proposed as an expectorant and bronchospasmolytic remedy for adults and in pediatry. As the hederasaponin C (HSC) is a chemical and biological marker of ivy, the simple and reliable HPLC method is developed for its quantitative evaluation in the thick extract. The UV detection is performed at 207 nm. The proposed HPLC method is linear in the range studied (r2>0.999) for all the analytes. The method is precise with intra- and inter-day variations of less than 2.0%. The mean recoveries of the analytes range from 96.7 to 99.2%. The results indicated that developed HPLC method could be used for the quality control of 'Kavsuron'